GPCR Division-Arrested Cells

GPCR Division-Arrested Cells

For Research Use Only. Not For Clinical Use.

Cells are the most critical and variable tools for GPCR functional cell-based assays. The quality and consistency are especially important for the robustness of high-throughput screening. To avoid the variability associated with cell division, division-arrested cells with improving consistency can be used as a substitute for dividing cells.

Figure 1. Comparison of dose-dependent inhibition curves in dividing (A) versus division-arrested (B) HEK293/secreted alkaline phosphatase (SEAP) cells in response to a panel of compounds. (Digan M E, et al., 2005)Figure 1. Comparison of dose-dependent inhibition curves in dividing (A) versus division-arrested (B) HEK293/secreted alkaline phosphatase (SEAP) cells in response to a panel of compounds. (Digan M E, et al., 2005)

GPCR division-arrested cells are derived from fully validated dividing stable cell lines treated with a low dose of mitomycin C. This treatment will not lead to apparent toxicity or obvious change to cellular signal transduction properties.

CD BioSciences provides a comprehensive portfolio of GPCR division-arrested cells that can be used to generate more consistent experimental results to help our customers speed up their process.

GPCR Division-Arrested CellsParental Cell List

We have successfully developed GPCR division-arrested cells in many types of parental cells, including but not limited to the following list.

1321N1 2F-2B 4T1 786O
A549 CHO H1299 HEK293
HEK293F HEK293T HepG2 Jurkat
MCF7 THP-1 U2OS V79

GPCR Division-Arrested CellsGPCR List

We have successfully developed GPCR division-arrested cells expressing various members of GPCR family, including but not limited to the following list.

Receptor Family Receptor Receptor Family Receptor
5-HT 5-HT1A Melanin-Concentrating Hormone MCH1
5-HT1B MCH2
5-HT2A Melanocortin MC1
5-HT2B MC2
5-HT3C MC3
5-HT4A MC4
5-HT4B Melatonin MT1
5-HT4D MT2
5-HT6 Metabotropic Glutamate mGlu1
5-HT7 mGlu2
Adenosine A1 mGlu3
A2A mGlu4
A2B mGlu5
A3 mGlu6
Adrenergic α1A mGlu7
α1B mGlu8
α1D Motilin Motilin
α2A Muscarinic M1
α2B M2
α2C M3
β1 M4
β2 M5
β3 Neuromedin U NMU1
Angiotensin AT1 NMU2
AT2 Neuropeptide NPBW1
Apelin APJ NPBW2
Bombesin BB1 NPFF1
BB2 NPFF2
BB3 NPS
Bradykinin B1 NPS(2)
B2 NPSIle107
Calcitonin AM1 Y1
AM2 Y2
AMY1 Y4
AMY2 Y5
AMY3 Neurotensin NTS1
CGRP NTS2
Calcium-Sensing CaSR Opioid δ Opioid
Cannabinoid CB1 κ Opioid
CB1a μ Opioid
CB1b NOP
CB2 Orexin OX1
Chemokine CCR1 OX2
CCR2 Orphan GPR3
CCR2b GPR119
CCR3 GPR139
CCR4 GPR142
CCR5 GPR151
CCR6 GPR17
CCR7 GPR18
CCR8 GPR183
CCR9 GPR27
CCR10 GPR34
CXCR1 GPR35
CXCR2 GPR37
CXCR3 GPR37L1
CXCR4 GPR39
CXCR5 GPR50
CXCR6 GPR55
CXCR7 GPR84
Cholecystokinin CCK1 GPR85
CCK2 MAS1
Corticotrophin-Releasing Factor CRF1 MRGX1
CRF2 MRGX2
Dopamine D1 Parathyroid Hormone PTH1
D2 PTH2
D3 Platelet-Activating Factor PAF
D4 Prokineticin PK1
D5 PK2
Endothelin ETA Prolactin-Releasing Peptide PrRP
ETB Prostanoid DP1
Formyl FPR1 DP2
FPR2 FP
Free Fatty Acid GPR40 IP1
GPR41 Prostaglandin E1
GPR43 Prostaglandin E2
GPR84 Prostaglandin E3
GPR119 Prostaglandin E4
GPR120 TP
GPR120L Protease-Activated PAR1
Galanin GAL1 PAR2
GAL2 PAR4
Ghrelin GHS-R1a Purinergic P2Y1
Glucagon GHRH P2Y2
GIP P2Y4
GLP-1 P2Y6
GLP-2 P2Y11
Gonadotrophin-Releasing Hormone GnRH P2Y12
Histamine H1 P2Y13
H2 P2Y14
H3 Relaxin RXFP1
H4 RXFP2
Hydroxycarboxylic Acid HCA1 RXFP3
HCA2 Somatostatin SST1
HCA3 SST2
Kisspeptin GPR54 SST3
Leukotriene BLT1 SST4
BLT2 SST5
CysLT1 Tachykinin NK1
CysLT2 NK2
Lysophospholipid LPA1 NK3
LPA2 Thyrotropin-Releasing Hormone TRH
LPA3 Trace Amine TA1
LPA4 Urotensin Urotensin-Ⅱ
LPA5 Vasoactive Intestinal Peptide PAC1
S1P1 VPAC1
S1P2 VPAC2
S1P3 Vasopressin V1A
S1P4 V1B
S1P5 V2

Advantages

  • Improved cell surface expression.
  • Comprehensive coverage of GPCR targets.
  • Flexible cell types and species.
  • Applicable to high-throughput screening with improving consistency.
  • Customized services to meet individual needs.
  • Available backup cell lines.
  • Competitive price and fast turnaround time.

CD BioSciences provides highly validated GPCR division-arrested cells to help our customers make GPCR research and drug discovery fast and standard. Our experienced scientists can customize GPCR division-arrested cells according to your specific needs to enable your discovery programs.

If you need more detailed information, please don't hesitate to contact us.

References

  1. Digan M E, et al. Evaluation of division-arrested cells for cell-based high-throughput screening and profiling. Journal of biomolecular screening, 2005, 10(6): 615-623.
  2. Fursov N, et al. Improving consistency of cell-based assays by using division-arrested cells. Assay and drug development technologies, 2005, 3(1): 7-15.