GPCR Division-Arrested Cells
Cells are the most critical and variable tools for GPCR functional cell-based assays. The quality and consistency are especially important for the robustness of high-throughput screening. To avoid the variability associated with cell division, division-arrested cells with improving consistency can be used as a substitute for dividing cells.
Figure 1. Comparison of dose-dependent inhibition curves in dividing (A) versus division-arrested (B) HEK293/secreted alkaline phosphatase (SEAP) cells in response to a panel of compounds. (Digan M E, et al., 2005)
GPCR division-arrested cells are derived from fully validated dividing stable cell lines treated with a low dose of mitomycin C. This treatment will not lead to apparent toxicity or obvious change to cellular signal transduction properties.
Creative Biolabs provides a comprehensive portfolio of GPCR division-arrested cells that can be used to generate more consistent experimental results to help our customers speed up their process.
Parental Cell List
We have successfully developed GPCR division-arrested cells in many types of parental cells, including but not limited to the following list.
| 1321N1 | 2F-2B | 4T1 | 786O |
| A549 | CHO | H1299 | HEK293 |
| HEK293F | HEK293T | HepG2 | Jurkat |
| MCF7 | THP-1 | U2OS | V79 |
GPCR List
We have successfully developed GPCR division-arrested cells expressing various members of GPCR family, including but not limited to the following list.
| Receptor Family | Receptor | Receptor Family | Receptor |
|---|---|---|---|
| 5-HT | 5-HT1A | Melanin-Concentrating Hormone | MCH1 |
| 5-HT1B | MCH2 | ||
| 5-HT2A | Melanocortin | MC1 | |
| 5-HT2B | MC2 | ||
| 5-HT3C | MC3 | ||
| 5-HT4A | MC4 | ||
| 5-HT4B | Melatonin | MT1 | |
| 5-HT4D | MT2 | ||
| 5-HT6 | Metabotropic Glutamate | mGlu1 | |
| 5-HT7 | mGlu2 | ||
| Adenosine | A1 | mGlu3 | |
| A2A | mGlu4 | ||
| A2B | mGlu5 | ||
| A3 | mGlu6 | ||
| Adrenergic | α1A | mGlu7 | |
| α1B | mGlu8 | ||
| α1D | Motilin | Motilin | |
| α2A | Muscarinic | M1 | |
| α2B | M2 | ||
| α2C | M3 | ||
| β1 | M4 | ||
| β2 | M5 | ||
| β3 | Neuromedin U | NMU1 | |
| Angiotensin | AT1 | NMU2 | |
| AT2 | Neuropeptide | NPBW1 | |
| Apelin | APJ | NPBW2 | |
| Bombesin | BB1 | NPFF1 | |
| BB2 | NPFF2 | ||
| BB3 | NPS | ||
| Bradykinin | B1 | NPS(2) | |
| B2 | NPSIle107 | ||
| Calcitonin | AM1 | Y1 | |
| AM2 | Y2 | ||
| AMY1 | Y4 | ||
| AMY2 | Y5 | ||
| AMY3 | Neurotensin | NTS1 | |
| CGRP | NTS2 | ||
| Calcium-Sensing | CaSR | Opioid | δ Opioid |
| Cannabinoid | CB1 | κ Opioid | |
| CB1a | μ Opioid | ||
| CB1b | NOP | ||
| CB2 | Orexin | OX1 | |
| Chemokine | CCR1 | OX2 | |
| CCR2 | Orphan | GPR3 | |
| CCR2b | GPR119 | ||
| CCR3 | GPR139 | ||
| CCR4 | GPR142 | ||
| CCR5 | GPR151 | ||
| CCR6 | GPR17 | ||
| CCR7 | GPR18 | ||
| CCR8 | GPR183 | ||
| CCR9 | GPR27 | ||
| CCR10 | GPR34 | ||
| CXCR1 | GPR35 | ||
| CXCR2 | GPR37 | ||
| CXCR3 | GPR37L1 | ||
| CXCR4 | GPR39 | ||
| CXCR5 | GPR50 | ||
| CXCR6 | GPR55 | ||
| CXCR7 | GPR84 | ||
| Cholecystokinin | CCK1 | GPR85 | |
| CCK2 | MAS1 | ||
| Corticotrophin-Releasing Factor | CRF1 | MRGX1 | |
| CRF2 | MRGX2 | ||
| Dopamine | D1 | Parathyroid Hormone | PTH1 |
| D2 | PTH2 | ||
| D3 | Platelet-Activating Factor | PAF | |
| D4 | Prokineticin | PK1 | |
| D5 | PK2 | ||
| Endothelin | ETA | Prolactin-Releasing Peptide | PrRP |
| ETB | Prostanoid | DP1 | |
| Formyl | FPR1 | DP2 | |
| FPR2 | FP | ||
| Free Fatty Acid | GPR40 | IP1 | |
| GPR41 | Prostaglandin E1 | ||
| GPR43 | Prostaglandin E2 | ||
| GPR84 | Prostaglandin E3 | ||
| GPR119 | Prostaglandin E4 | ||
| GPR120 | TP | ||
| GPR120L | Protease-Activated | PAR1 | |
| Galanin | GAL1 | PAR2 | |
| GAL2 | PAR4 | ||
| Ghrelin | GHS-R1a | Purinergic | P2Y1 |
| Glucagon | GHRH | P2Y2 | |
| GIP | P2Y4 | ||
| GLP-1 | P2Y6 | ||
| GLP-2 | P2Y11 | ||
| Gonadotrophin-Releasing Hormone | GnRH | P2Y12 | |
| Histamine | Histamine1 | P2Y13 | |
| Histamine2 | P2Y14 | ||
| Histamine3 | Relaxin | RXFP1 | |
| Histamine4 | RXFP2 | ||
| Hydroxycarboxylic Acid | HCA1 | RXFP3 | |
| HCA2 | Somatostatin | SST1 | |
| HCA3 | SST2 | ||
| Kisspeptin | GPR54 | SST3 | |
| Leukotriene | BLT1 | SST4 | |
| BLT2 | SST5 | ||
| CysLT1 | Tachykinin | NK1 | |
| CysLT2 | NK2 | ||
| Lysophospholipid | LPA1 | NK3 | |
| LPA2 | Thyrotropin-Releasing Hormone | TRH | |
| LPA3 | Trace Amine | TA1 | |
| LPA4 | Urotensin | Urotensin-Ⅱ | |
| LPA5 | Vasoactive Intestinal Peptide | PAC1 | |
| S1P1 | VPAC1 | ||
| S1P2 | VPAC2 | ||
| S1P3 | Vasopressin | V1A | |
| S1P4 | V1B | ||
| S1P5 | V2 |
Advantages
- Improved cell surface expression.
- Comprehensive coverage of GPCR targets.
- Flexible cell types and species.
- Applicable to high-throughput screening with improving consistency.
- Customized services to meet individual needs.
- Available backup cell lines.
- Competitive price and fast turnaround time.
Creative Biolabs provides highly validated GPCR division-arrested cells to help our customers make GPCR research and drug discovery fast and standard. Our experienced scientists can customize GPCR division-arrested cells according to your specific needs to enable your discovery programs.
If you need more detailed information, please don't hesitate to contact us.
References
- Digan M E, et al. Evaluation of division-arrested cells for cell-based high-throughput screening and profiling. Journal of biomolecular screening, 2005, 10(6): 615-623.
- Fursov N, et al. Improving consistency of cell-based assays by using division-arrested cells. Assay and drug development technologies, 2005, 3(1): 7-15.