GPCR Frozen Cells
G protein-coupled receptors (GPCRs) play critical roles in the transduction of extracellular stimuli into intracellular signals. They can respond to multiple stimuli and also be modulated by various cellular signals. GPCRs are involved in multiple physiological activities and are related to various diseases. They are a major family of drug targets.
Cell-based assays have an important role in GPCR research and drug discovery. However, cell condition is the key determinant for successful cell-based assays. Frozen cells can contribute to decrease variations of experimental results, especially during the initial cell-based screening process.
Creative Biolabs provides a comprehensive portfolio of GPCR frozen cells to help our customers perform faster cell-based assays with improved consistency. Our ready-to-use GPCR frozen cells can be used to accelerate data generation.
Parental Cell List
We can provide GPCR frozen cells generated from many types of parental cells, including but not limited to the following list.
| 1321N1 | 2F-2B | 4T1 | 786O |
| A549 | CHO | H1299 | HEK293 |
| HEK293F | HEK293T | HepG2 | Jurkat |
| MCF7 | THP-1 | U2OS | V79 |
GPCR List
We can offer GPCR frozen cells expressing various members of GPCR family, including but not limited to the following list.
| Receptor Family | Receptor | Receptor Family | Receptor |
|---|---|---|---|
| 5-HT | 5-HT1A | Melanin-Concentrating Hormone | MCH1 |
| 5-HT1B | MCH2 | ||
| 5-HT2A | Melanocortin | MC1 | |
| 5-HT2B | MC2 | ||
| 5-HT3C | MC3 | ||
| 5-HT4A | MC4 | ||
| 5-HT4B | Melatonin | MT1 | |
| 5-HT4D | MT2 | ||
| 5-HT6 | Metabotropic Glutamate | mGlu1 | |
| 5-HT7 | mGlu2 | ||
| Adenosine | A1 | mGlu3 | |
| A2A | mGlu4 | ||
| A2B | mGlu5 | ||
| A3 | mGlu6 | ||
| Adrenergic | α1A | mGlu7 | |
| α1B | mGlu8 | ||
| α1D | Motilin | Motilin | |
| α2A | Muscarinic | M1 | |
| α2B | M2 | ||
| α2C | M3 | ||
| β1 | M4 | ||
| β2 | M5 | ||
| β3 | Neuromedin U | NMU1 | |
| Angiotensin | AT1 | NMU2 | |
| AT2 | Neuropeptide | NPBW1 | |
| Apelin | APJ | NPBW2 | |
| Bombesin | BB1 | NPFF1 | |
| BB2 | NPFF2 | ||
| BB3 | NPS | ||
| Bradykinin | B1 | NPS(2) | |
| B2 | NPSIle107 | ||
| Calcitonin | AM1 | Y1 | |
| AM2 | Y2 | ||
| AMY1 | Y4 | ||
| AMY2 | Y5 | ||
| AMY3 | Neurotensin | NTS1 | |
| CGRP | NTS2 | ||
| Calcium-Sensing | CaSR | Opioid | δ Opioid |
| Cannabinoid | CB1 | κ Opioid | |
| CB1a | μ Opioid | ||
| CB1b | NOP | ||
| CB2 | Orexin | OX1 | |
| Chemokine | CCR1 | OX2 | |
| CCR2 | Orphan | GPR3 | |
| CCR2b | GPR119 | ||
| CCR3 | GPR139 | ||
| CCR4 | GPR142 | ||
| CCR5 | GPR151 | ||
| CCR6 | GPR17 | ||
| CCR7 | GPR18 | ||
| CCR8 | GPR183 | ||
| CCR9 | GPR27 | ||
| CCR10 | GPR34 | ||
| CXCR1 | GPR35 | ||
| CXCR2 | GPR37 | ||
| CXCR3 | GPR37L1 | ||
| CXCR4 | GPR39 | ||
| CXCR5 | GPR50 | ||
| CXCR6 | GPR55 | ||
| CXCR7 | GPR84 | ||
| Cholecystokinin | CCK1 | GPR85 | |
| CCK2 | MAS1 | ||
| Corticotrophin-Releasing Factor | CRF1 | MRGX1 | |
| CRF2 | MRGX2 | ||
| Dopamine | D1 | Parathyroid Hormone | PTH1 |
| D2 | PTH2 | ||
| D3 | Platelet-Activating Factor | PAF | |
| D4 | Prokineticin | PK1 | |
| D5 | PK2 | ||
| Endothelin | ETA | Prolactin-Releasing Peptide | PrRP |
| ETB | Prostanoid | DP1 | |
| Formyl | FPR1 | DP2 | |
| FPR2 | FP | ||
| Free Fatty Acid | GPR40 | IP1 | |
| GPR41 | Prostaglandin E1 | ||
| GPR43 | Prostaglandin E2 | ||
| GPR84 | Prostaglandin E3 | ||
| GPR119 | Prostaglandin E4 | ||
| GPR120 | TP | ||
| GPR120L | Protease-Activated | PAR1 | |
| Galanin | GAL1 | PAR2 | |
| GAL2 | PAR4 | ||
| Ghrelin | GHS-R1a | Purinergic | P2Y1 |
| Glucagon | GHRH | P2Y2 | |
| GIP | P2Y4 | ||
| GLP-1 | P2Y6 | ||
| GLP-2 | P2Y11 | ||
| Gonadotrophin-Releasing Hormone | GnRH | P2Y12 | |
| Histamine | Histamine1 | P2Y13 | |
| Histamine2 | P2Y14 | ||
| Histamine3 | Relaxin | RXFP1 | |
| Histamine4 | RXFP2 | ||
| Hydroxycarboxylic Acid | HCA1 | RXFP3 | |
| HCA2 | Somatostatin | SST1 | |
| HCA3 | SST2 | ||
| Kisspeptin | GPR54 | SST3 | |
| Leukotriene | BLT1 | SST4 | |
| BLT2 | SST5 | ||
| CysLT1 | Tachykinin | NK1 | |
| CysLT2 | NK2 | ||
| Lysophospholipid | LPA1 | NK3 | |
| LPA2 | Thyrotropin-Releasing Hormone | TRH | |
| LPA3 | Trace Amine | TA1 | |
| LPA4 | Urotensin | Urotensin-Ⅱ | |
| LPA5 | Vasoactive Intestinal Peptide | PAC1 | |
| S1P1 | VPAC1 | ||
| S1P2 | VPAC2 | ||
| S1P3 | Vasopressin | V1A | |
| S1P4 | V1B | ||
| S1P5 | V2 |
Advantages
- Hassle-free – Directly thaw into proper assay plate without the need for extensive cell culture maintenance.
- Fast – Results will be obtained within 24 hours using ready-to-use frozen cells.
- High quality – High cellular viability and better signal-to-noise ratio.
- Highly validated – Cells with high functional expression of GPCRs have been functionally validated and tested under variable conditions.
- Applicable to high-throughput screening with improving consistency.
- Available backup cell lines.
- Competitive price and fast turnaround time.
Creative Biolabs provides highly validated GPCR frozen cells to help our customers make GPCR research and drug discovery fast and easy. Our experienced scientists can customize GPCR frozen cells according to your specific needs to enable your discovery programs. We also offer frozen cell products for your choice.
If you need more detailed information, please don't hesitate to contact us.
Reference
- Noh H & Lee S. Optimization of the cryopreserved condition for utilization of GPCR frozen cells. Journal of the Korea Academia-Industrial cooperation Society, 2015, 16(2): 1200-1206.