Specification

• Cell Line:
• CHO-K1

• GPCR Family:
• Orexin Receptors

• Gene Name:
• HCRTR2

• GenBank Accession Number:
• NM_001526.2

• Cell State:
• Division-Arrested Cells

• Detection Method:
• Fluorescent

• Component:
• This assay kit includes sufficient cells and substrate to analyze 1 × 384-well plate

• Shipping Condition:
• Dry Ice

• Storage:
• Store in liquid nitrogen immediately upon receipt or thaw for immediate use.

• Population Status:
• Single Cell Clone Origin

• Receptor Type:
• BLA (Beta-Lactamase)

Description

• CHO-K1/NFAT-bla/HCRTR2 is a stable recombinant human cell line expressing the hhuman Hypocretin 2 receptor (HCRTR2). Under the control of the Nuclear Factor of Activated T-cells (NFAT) response element and the Promiscuous G protein, Gα15, this cell line can stably express the beta-lactamase (bla) reporter gene. Using a low-dose treatment of Mitomycin-C, these cells are irreversibly division arrested, but without apparent toxicity or change in cellular signal transduction. These cells have been functionally validated and tested under variable conditions. The HCRTR2 receptor is expressed in brain, adrenal gland, pancreas and gastrointestinal tract. It is implicated in narcolepsy.