Specification

• Cell Line:
• HEK293T

• GPCR Family:
• Lysophosphatidic Acid (LPA) Receptors

• Gene Name:
• EDG3

• GenBank Accession Number:
• NM_005226.2

• Cell State:
• Division-Arrested Cells

• Detection Method:
• Fluorescent

• Component:
• This assay kit includes sufficient cells and substrate to analyze 1 × 384-well plate

• Shipping Condition:
• Dry Ice

• Storage:
• Store in liquid nitrogen immediately upon receipt or thaw for immediate use.

• Population Status:
• Single Cell Clone Origin

• Receptor Type:
• BLA (Beta-Lactamase)

Description

• HEK293T/Gα15-NFAT-bla/EDG3 is a stable recombinant human cell line expressing the human Endothelial Differentiation Receptor 3 (EDG3). Under the control of the Nuclear Factor of Activated T-cells (NFAT) response element and the Promiscuous G protein, Gα15, this cell line can stably express the beta-lactamase (bla) reporter gene. Using a low-dose treatment of Mitomycin-C, these cells are irreversibly division arrested, but without apparent toxicity or change in cellular signal transduction. These cells have been functionally validated and tested under variable conditions. EDG3 or Sphingosine 1-phosphate receptor 3 (S1P3), which is a Gq/Gi/Go/Gα13/Gα12 coupled GPCR, has been shown identified as a functional receptor for sphingosine 1-phosphate and likely contributes to the regulation of angiogenesis and vascular endothelial cell function.