U2OS/EDG6-bla Division Arrested Assay Kit
For Research Use Only. Not For Clinical Use.
Catalog Number: DAC0034
Product Size | Price |
---|---|
1 kit | Online Inquiry |
Specification
- Cell Line:
- U2OS
- GPCR Family:
- Lysophosphatidic Acid (LPA) Receptors
- Gene Name:
- EDG6
- GenBank Accession Number:
- NM_003775.1
- Cell State:
- Division-Arrested Cells
- Detection Method:
- Fluorescent
- Component:
- This assay kit includes sufficient cells and substrate to analyze 1 × 384-well plate
- Shipping Condition:
- Dry Ice
- Storage:
- Should be stored in liquid nitrogen immediately upon receipt.
- Population Status:
- Pool of Stable Cells
- Receptor Type:
- BLA (Beta-Lactamase)
- Cytoplasmic Tail:
- Tail from GRPR GPCR
Description
- U2OS/EDG6-bla is a stable recombinant human cell line expressing the human Endothelial Differentiation, Lysophosphatidic Acid G-protein-coupled Receptor, 6 (EDG6) linked to a TEV protease site and a Gal4-VP16 transcription factor. Under the control of a UAS response element, this cell line can stably express a beta-arrestin/TEV protease fusion protein and the beta-lactamase (bla) reporter gene. Using a low-dose treatment of Mitomycin-C, these cells are irreversibly division arrested, but without apparent toxicity or change in cellular signal transduction. These cells have been functionally validated and tested under variable conditions.