U2OS/EDG6-bla Division Arrested Assay Kit

U2OS/EDG6-bla Division Arrested Assay Kit

For Research Use Only. Not For Clinical Use.

Catalog Number: DAC0034

Product Size Price
1 kit Online Inquiry

Specification

  • Cell Line:
  • U2OS
  • GPCR Family:
  • Lysophosphatidic Acid (LPA) Receptors
  • Gene Name:
  • EDG6
  • GenBank Accession Number:
  • NM_003775.1
  • Cell State:
  • Division-Arrested Cells
  • Detection Method:
  • Fluorescent
  • Component:
  • This assay kit includes sufficient cells and substrate to analyze 1 × 384-well plate
  • Shipping Condition:
  • Dry Ice
  • Storage:
  • Should be stored in liquid nitrogen immediately upon receipt.
  • Population Status:
  • Pool of Stable Cells
  • Receptor Type:
  • BLA (Beta-Lactamase)
  • Cytoplasmic Tail:
  • Tail from GRPR GPCR

Description

  • U2OS/EDG6-bla is a stable recombinant human cell line expressing the human Endothelial Differentiation, Lysophosphatidic Acid G-protein-coupled Receptor, 6 (EDG6) linked to a TEV protease site and a Gal4-VP16 transcription factor. Under the control of a UAS response element, this cell line can stably express a beta-arrestin/TEV protease fusion protein and the beta-lactamase (bla) reporter gene. Using a low-dose treatment of Mitomycin-C, these cells are irreversibly division arrested, but without apparent toxicity or change in cellular signal transduction. These cells have been functionally validated and tested under variable conditions.