Fluo-4 No-Wash Calcium Assay Kit

Fluo-4 No-Wash Calcium Assay Kit

For Research Use Only. Not For Clinical Use.

Catalog Number: ION0028

Product Size Price
10 microplates Online Inquiry
100 microplates Online Inquiry

Specification

  • Detection Method:
  • Fluorescent
  • Component:
  • • Fluo-4 No-Wash Dye Mix
    • Probenecid, water soluble
    • Assay buffer
  • Shipping Condition:
  • Room Temperature
  • Storage:
  • Store in freezer (-5 to -30°C) and protect from light.
  • Cell Permeability:
  • Cell-Permeant
  • Applicable Equipment:
  • Microplate Reader
  • Indicator Type:
  • Calcium Indicator
  • Indicator Properties:
  • Fluorescent Dye-Based

Description

  • Fluo-4 No-Wash Calcium Assay Kit, which requires neither a wash step nor a quencher dye, achieves larger increases in fluorescence intensity, lower variability and higher Z´ values. The indicator in this kit is nonfluorescent and stable in pH 7–7.5 buffer for several hours. The background fluorescence of the growth medium can be eliminated by removing the medium prior to adding the indicator dye to the wells. This kit provides an easier and faster assay.