Specification

• Caterogy:
• Enzyme Substrates

• Cell Permeability:
• Cell-Permeant

• Detection Method:
• Fluorescent

• Applicable Equipment:
• Flow Cytometer, Microplate Reader

• Shipping Condition:
• Room Temperature

• Storage:
• Store in freezer (-5 to -30°C).

• Substrate:
• CCF4-AM

• Substrate Properties:
• Chemical Substrate

• Substrate Type:
• Beta-Lactamase Substrate

Description

• CCF4-AM is a lipophilic, esterified form of CCF4 substrate and can readily enter cells. CCF4-AM can be cleavaged by endogenous cytoplasmic esterases and rapidly converted into CCF4, its negatively charged form, to retain in the cytosol. CCF4, a fluorescence resonance energy transfer (FRET) substrate, consists of a cephalosporin core linking 7-hydroxycoumarin to Frescein and can be used as a sensitive reporter for β-lactamase of mammalian gene expression.