Beta-lactamase substrates, CCF4-AM

Beta-lactamase substrates, CCF4-AM

For Research Use Only. Not For Clinical Use.

Catalog Number: ENZ0003

Product Size Price
5 mg Online Inquiry
20 mg Online Inquiry


  • Caterogy:
  • Enzyme Substrates
  • Cell Permeability:
  • Cell-Permeant
  • Detection Method:
  • Fluorescent
  • Applicable Equipment:
  • Flow Cytometer, Microplate Reader
  • Shipping Condition:
  • Room Temperature
  • Storage:
  • Store in freezer (-5 to -30°C).
  • Substrate:
  • CCF4-AM
  • Substrate Properties:
  • Chemical Substrate
  • Substrate Type:
  • Beta-Lactamase Substrate


  • CCF4-AM is a lipophilic, esterified form of CCF4 substrate and can readily enter cells. CCF4-AM can be cleavaged by endogenous cytoplasmic esterases and rapidly converted into CCF4, its negatively charged form, to retain in the cytosol. CCF4, a fluorescence resonance energy transfer (FRET) substrate, consists of a cephalosporin core linking 7-hydroxycoumarin to Frescein and can be used as a sensitive reporter for β-lactamase of mammalian gene expression.