Specification

• Caterogy:
• Ion Indicators

• Cell Permeability:
• Cell-Impermeant

• CAS Number:
• N/A

• Molecular Formula:
• C35H21F4K5N2O13

• Molecular Weight (kDa):
• 949.0416

• Detection Method:
• Fluorescent

• Applicable Equipment:
• Confocal Microscope, Fluorescence Microscope, Fluorescent Imager, HTS Reader, High Content Instrument, Microplate Reader

• Shipping Condition:
• Room Temperature

• Storage:
• Store in freezer (-5 to -30°C) and protect from light.

• Indicator Properties:
• Fluorescent Dye-Based

• Indicator Type:
• Calcium Indicator

Description

• Labeled calcium indicators can exhibit an increase in fluorescence upon binding Ca²⁺. The fluorescence intensity can increase more than 100 fold upon binding Ca²⁺. Fluo-4FF is an analog of Fluo-4 with lower Ca²⁺-binding affinity. It is compatible with excitation at 488 nm by argon-ion laser sources and suitable for detecting intracellular calcium levels in the 1 µM to 1 mM range. The heavy metal-selective chelator TPEN can be used to control the perturbations of heavy metal cations. This cell impermeant indicators can be physically loaded using patch pipette, microinjection, or pinocytotic cell-loading reagent.

Detailed Information

• • Ex/Em of Ca²⁺–bound form: 494/516 nm
  • Kd for Ca²⁺ in buffer: ~9.7 µM