Fluo-3, AM, ExtraPure grade

Fluo-3, AM, ExtraPure grade

For Research Use Only. Not For Clinical Use.

Catalog Number: ION0014

CAS Number: 121714-22-5

Product Size Price
10 × 50 µg Online Inquiry


  • Caterogy:
  • Ion Indicators
  • Cell Permeability:
  • Cell-Permeant
  • CAS Number:
  • 121714-22-5
  • Molecular Formula:
  • C51H50Cl2N2O23
  • Molecular Weight (kDa):
  • 1129.8616
  • Detection Method:
  • Fluorescent
  • Applicable Equipment:
  • Confocal Microscope, Fluorescence Microscope, Fluorescent Imager, HTS Reader, High Content Instrument, Microplate Reader
  • Shipping Condition:
  • Room Temperature
  • Storage:
  • Store in freezer (-5 to -30°C) and protect from light.
  • Indicator Properties:
  • Fluorescent Dye-Based
  • Indicator Type:
  • Calcium Indicator


  • Labeled calcium indicators can exhibit an increase in fluorescence upon binding Ca2+. The fluorescence intensity can increase more than 100 fold upon binding Ca2+. Fura-2 AM, ExtraPure is greater than or equal to 98% pure by HPLC. Fluo-3, AM is a cell permeable Ca2+ indicator that can be activated by visible light. It has a high affinity. Fluo-3 can be used in the imagination of the Ca2+ signaling spatial dynamics and cell-based pharmacological screening. It can be used in flow cytometry experiments for the detection of second messengers, neurotransmitters and photoactivation of caged chelators. The heavy metal-selective chelator TPEN can be used to control the perturbations of heavy metal cations. This cell impermeant indicator can be physically loaded using patch pipette, microinjection, or pinocytotic cell-loading reagent.

Detailed Information

  • • Ex/Em of Ca2+–bound form: 506/526 nm
    • Kd for Ca2+ in buffer: ~335 nM