Fluo-3, AM, ExtraPure grade
For Research Use Only. Not For Clinical Use.
Specification
- Caterogy:
- Ion Indicators
- Cell Permeability:
- Cell-Permeant
- CAS Number:
- 121714-22-5
- Molecular Formula:
- C51H50Cl2N2O23
- Molecular Weight (kDa):
- 1129.8616
- Detection Method:
- Fluorescent
- Applicable Equipment:
- Confocal Microscope, Fluorescence Microscope, Fluorescent Imager, HTS Reader, High Content Instrument, Microplate Reader
- Shipping Condition:
- Room Temperature
- Storage:
- Store in freezer (-5 to -30°C) and protect from light.
- Indicator Properties:
- Fluorescent Dye-Based
- Indicator Type:
- Calcium Indicator
Description
- Labeled calcium indicators can exhibit an increase in fluorescence upon binding Ca2+. The fluorescence intensity can increase more than 100 fold upon binding Ca2+. Fura-2 AM, ExtraPure is greater than or equal to 98% pure by HPLC. Fluo-3, AM is a cell permeable Ca2+ indicator that can be activated by visible light. It has a high affinity. Fluo-3 can be used in the imagination of the Ca2+ signaling spatial dynamics and cell-based pharmacological screening. It can be used in flow cytometry experiments for the detection of second messengers, neurotransmitters and photoactivation of caged chelators. The heavy metal-selective chelator TPEN can be used to control the perturbations of heavy metal cations. This cell impermeant indicator can be physically loaded using patch pipette, microinjection, or pinocytotic cell-loading reagent.
Detailed Information
-
• Ex/Em of Ca2+–bound form: 506/526 nm
• Kd for Ca2+ in buffer: ~335 nM