Specification

• Caterogy:
• Ion Indicators

• Cell Permeability:
• Cell-Permeant

• Concentration:
• 1 mM

• CAS Number:
• 273221-67-3

• Molecular Formula:
• C51H50F2N2O23

• Molecular Weight (kDa):
• 1096.95

• Detection Method:
• Fluorescent

• Applicable Equipment:
• Confocal Microscope, Fluorescence Microscope, Fluorescent Imager, HTS Reader, High Content Instrument, Microplate Reader

• Shipping Condition:
• Room Temperature

• Storage:
• Store in freezer (-5 to -30°C) and protect from light.

• Indicator Properties:
• Fluorescent Dye-Based

• Indicator Type:
• Calcium Indicator

Description

• Labeled calcium indicators can exhibit an increase in fluorescence upon binding Ca²⁺. The fluorescence intensity can increase more than 100 fold upon binding Ca²⁺. Fluo-4 is an analog of Fluo-3 with the two chlorine substituents replaced by fluorines. It results in increased fluorescence excitation at 488 nm and consequently higher fluorescence signal levels. The heavy metal-selective chelator TPEN can be used to control the perturbations of heavy metal cations. This cell impermeant indicators can be physically loaded using patch pipette, microinjection, or pinocytotic cell-loading reagent.

Detailed Information

• • Ex/Em of Ca²⁺–bound form: 494/516 nm
  • Kd for Ca²⁺ in buffer: ~335 nM