Fluo-4, AM

Fluo-4, AM

For Research Use Only. Not For Clinical Use.

Catalog Number: ION0015

CAS Number: 273221-67-3

Product Size Price
500 µL Online Inquiry


  • Caterogy:
  • Ion Indicators
  • Cell Permeability:
  • Cell-Permeant
  • Concentration:
  • 1 mM
  • CAS Number:
  • 273221-67-3
  • Molecular Formula:
  • C51H50F2N2O23
  • Molecular Weight (kDa):
  • 1096.95
  • Detection Method:
  • Fluorescent
  • Applicable Equipment:
  • Confocal Microscope, Fluorescence Microscope, Fluorescent Imager, HTS Reader, High Content Instrument, Microplate Reader
  • Shipping Condition:
  • Room Temperature
  • Storage:
  • Store in freezer (-5 to -30°C) and protect from light.
  • Indicator Properties:
  • Fluorescent Dye-Based
  • Indicator Type:
  • Calcium Indicator


  • Labeled calcium indicators can exhibit an increase in fluorescence upon binding Ca2+. The fluorescence intensity can increase more than 100 fold upon binding Ca2+. Fluo-4 is an analog of Fluo-3 with the two chlorine substituents replaced by fluorines. It results in increased fluorescence excitation at 488 nm and consequently higher fluorescence signal levels. The heavy metal-selective chelator TPEN can be used to control the perturbations of heavy metal cations. This cell impermeant indicators can be physically loaded using patch pipette, microinjection, or pinocytotic cell-loading reagent.

Detailed Information

  • • Ex/Em of Ca2+–bound form: 494/516 nm
    • Kd for Ca2+ in buffer: ~335 nM