Cell-Based GPCR Assays

G protein-coupled receptors (GPCRs), also known as seven-(pass)-transmembrane domain receptors, heptahelical receptors or 7-TM receptors, mediate various important physiological functions. They are considered as the most important drug targets for a broad spectrum of diseases. With the development of assay technologies and methodologies, GPCR drug development has achieved further success.

Compared to cell-free assays, cell-based assays can more accurately characterize the cellular behavior in response to stimulation and offer more useful information under conditions closer to the physiological environment. These benefits contribute to the increasing use of cell-based assays in GPCR research and drug development.

Creative Biolabs offers a comprehensive selection of cell-based GPCR assays to meet the requirements of both GPCR research and drug development. With advanced techniques, state-of-the-art platforms and experienced scientists, we are your ideal partner to accelerate your project lifecycle.

Cell-Based GPCR Reporter Assays

The activation of GPCRs can induce the transcription of downstream genes by initiating a cascade of second messenger pathways. Reporter assays provide an easy way to measure the activation of a specific signaling cascade of interests and a simple high throughput method for screening GPCR targets linked to second messenger pathways.

We provide a number of cell-based GPCR reporter systems for different applications. We have also developed reporter assays for live cell imaging.

Cell-Based Second Messenger Assays

Second messengers are one of the initiating components of intracellular signal transduction. The release of second messengers is response to the signal molecules exposed outside cells. The binding of external signaling molecules to GPCRs cause a conformational change, which results in the interaction between GPCRs and G proteins, and in turn triggers the production of hundreds or even thousands of second messenger molecules.

We provide various assays for the detection and quantification of different second messengers to elucidate second messenger signaling cascades resulting from interactions between GPCRs and their ligands.

Label-Free Cell-Based Assays

Label-free cell-based assays, which can capture the integrated cell response, has become a very active area for GPCR screening. Biosensors are generally employed in label-free cell-based assays to monitor cell response and can monitor the activity of different classes of GPCRs simultaneously. These assays can further strengthen the role of GPCRs in drug development.

We provide both resonant waveguide grating (RWG) biosensor systems and electrical biosensor systems for label-free cell-based assays available for GPCR screening.

Our Advantages

• Professional scientific team with extensive experience.
• Various options for maximum flexibility to meet specific requirements.
• The most versatile and relevant assays for specific purpose.
• Effective problem-solving process and ongoing support to accelerate your project.
• Fast turnaround time and competitive price

If you are interested in our cell-based GPCR assays or have any specific needs, please feel free to contact us for more details.