Protein Labeling of GPCR
Creative Biolabs provides high-quality protein labeling services for both GPCR research and pharmaceutical use. With technical expertise and facilities dedicated to protein labeling, we can help our customers save time and effort.
Introduction of Protein Labeling
Molecular labels can be used to monitor biological processes, facilitate the detection of labeled proteins, or purify labeled proteins and their binding partners. Protein labeling can improve detection sensitivity and simplify the process. Different labeling strategies results in the covalent attachment of different types of protein labels, such as biotin, enzyme conjugates, and fluorescent probes, to target proteins. It is necessary to carefully consider the type of labeling strategy and label tailored for different applications.
G protein-coupled receptors (GPCRs), which mediate a wide range of physiological responses, serve as an important category of drug targets. Protein labeling is essential to study the abundance, distribution, structure, conformational changes, protein interaction, internalization process, and post-translational modification of GPCRs. It can also help gain mechanistic insight of GPCR functions.
Figure 1. Labeling techniques for G protein-coupled receptors modified at their N-terminus. (Cottet M, et al., 2011)
Our Services
We can provide our customers with the widest labeling options to fully meet their specific requirements. Various types of protein labels and labeling strategies are available to meet different needs of biological and pharmaceutical applications.
Our experienced scientists can choose the most appropriate labeling strategy to ensure that the final choice is fully in line with the intended downstream application and help achieve the desired results.
Most Common Choices of Protein Labels
| Protein Labels | Description |
|---|---|
| Biotin | Biotin, also known as vitamin H or vitamin B7, exhibits an extraordinary binding affinity for avidin and streptavidin. It is a useful label for the detection, purification and immobilization of target proteins. |
| Enzyme conjugates | Some enzymes conjugation with proteins can be used as highly sensitive probes for the detection of proteins in whole cells, lysates or tissues. Horseradish peroxidase (HRP), alkaline phosphatase (AP), glucose oxidase and β-galactosidase are commonly used enzymes for protein labeling. |
| Fluorescent labels | Biological fluorophores are suitable to chemically label or stain most proteins. Fluorescent labels can be used in various applications, such as cell imaging, flow cytometry, and high-content analysis. Organic dyes and quantum dots are commonly used. |
| Stable isotopes | Stable isotopes, non-radioactive forms of atoms, can be used to label peptides and proteins by chemical or enzymatic reactions. Stable isotopic labeling is a powerful tool for quantitative proteomics. |
Diverse Choices of Labeling Strategies
| Labeling Strategies | Description |
|---|---|
| In vitro protein labeling | In vitro protein labeling strategies generally involve the covalent attachment of labels to proteins. These strategies are applicable to a variety of applications, such as the studies on structures and functions of proteins under in vitro conditions. |
| In vivo protein labeling | In vivo protein labeling strategies play an important role in the study of protein distribution, interaction and function under physiological conditions. |
| Single-molecule methods | Single-molecule techniques are powerful tools to understand the dynamic signaling and conformational complexity of GPCRs at single-molecule level. |
| Site-specific protein labeling | Site-specific protein labeling using fluorophores and other moieties is a robust method that permits the development of a variety of assays for proteomic analysis. |
Our Advantages
- Comprehensive list of protein labels.
- Best solutions based on diverse choices of labeling strategies.
- High quality results that meet specific requirements.
- High efficiency and cost-saving services.
Creative Biolabs is always ready to provide the best services to each of our customers. We can help you choose the appropriate labeling strategies and offer the optimized protocols.
If you are interested in our services or have any specific requirements, please feel free to contact us for more details.
References
- Cottet M, et al. Original fluorescent ligand-based assays open new perspectives in G protein-coupled receptors drug screening. Pharmaceuticals, 2011, 4(1): 202-214.
- Jiang W X, et al. Specific cell surface labeling of GPCRs using split GFP. Scientific reports, 2016, 6: 20568.
- Tian H, et al. Labeling and single-molecule methods to monitor G protein-coupled receptor dynamics. Chemical reviews, 2017, 117(1): 186-245.