Genetic Polymorphism of GPCRs
Creative Biolabs provides professional services for the study of GPCR polymorphisms to help our customers accelerate their basic research and drug development process.
Introduction of Genetic Polymorphisms
Genetic polymorphisms refer to the existence of more than one allele in a single genetic locus within a population, and the least common allele has a frequency of about 1% or greater. It is the difference in DNA sequence among individuals, groups or populations, and can occur in any region of the genome. Genetic polymorphisms have been shown to be associated with various diseases.
G protein-coupled receptors (GPCRs), the most diverse group of membrane receptors in eukaryotes, play an important role in signal recognition and transduction. They are involved in the regulation of various physiological processes. Gene polymorphisms of GPCRs have been shown to cause a variety of diseases. The study of GPCR polymorphisms will have a significant impact on medicine and pharmacology.
A variety of techniques are available at Creative Biolabs to meet different requirements.
- Restriction Fragment Length Polymorphism
- Amplication Fragment Length Polymorphism
- Dynamic Allele-Specific Hybridization
- Single Nucleotide Polymorphism Microarray
- Molecular Beacons
- Single Strand Conformation Polymorphism
- Denaturing Gradient Gel Electrophoresis
- Temperature Gradient Gel Electrophoresis
RFLP is a molecular technique to detect variations in homologous DNA sequence recognized by restriction enzymes. It is an important tool for genome mapping and the investigation of genetic diseases.
AFLP is a PCR-based technique for detecting DNA polymorphism when genome information is unknown. It can be used to simultaneously detect various polymorphisms in different genomic regions.
DASH is capable to measure the quantifiable change in melting temperature (Tm) caused by the instability of mismatched base pairs. It can be used to measure all types of mutations.
Hundreds of thousands of probes arrayed on single nucleotide polymorphism (SNP) microarray allow the simultaneously detection of many SNPs. It can also be used to characterize genetic diversity and identify new drug targets.
Molecular beacons are specifically engineered single-stranded oligonucleotide hybridizing probes. They can be employed for the detection of SNPs, and the unique design allows to identify SNPs at a given location.
Differences in single-stranded nucleotide sequences of identical length can induce conformational differences. SSCP can be used to distinguish these sequence variations through electrophoretic mobility differences.
DGGE can be used as an alternative of RFLP to identify DNA polymorphism. It allows the detection of changes as small as a single base-pair difference between two DNA fragments.
TGGE is a technology developed on the bases of DGGE. The chemical denaturant used in DGGE is replaced by temperature gradient. TGGE can be used to detect polymorphic DNA and is possible to screen large numbers of samples.
Creative Biolabs provides customized GPCR mutagenesis services in a fast and cost-saving way. If you are interested in our services or have any specific needs, please feel free to contact us for more details.
- Rana B K, et al. Genetic variations and polymorphisms of G protein-coupled receptors: functional and therapeutic implications. Annual review of pharmacology and toxicology, 2001, 41(1): 593-624.
- Sadee W, et al. Genetic variations in human G protein-coupled receptors: implications for drug therapy. AAPs PharmSci, 2001, 3(3): 54-80.