GPCRs Single Molecule Analysis

GPCRs Single Molecule Analysis

For Research Use Only. Not For Clinical Use.

CD BioSciences provides state-of-the-art single molecule analysis of GPCRs to help advance cutting-edge GPCR research and drug development projects.

Introduction of Single Molecule Analysis

The basic function of life unit mainly depends on a single macromolecule. The measurement on a bulk collection of molecules can only measure the average characteristics, but cannot distinguish individual behavior. In the past few decades, technological breakthroughs have made it possible to analyze single molecules.  Single molecule analysis allows the measurement of individual properties. These technologies are increasingly used in cell biology, molecular biology, biochemistry and biophysics.

G protein-coupled receptors (GPCRs) constitute the most important pharmacological targets. The unprecedented spatial and temporal resolution of single molecule analysis and its ability to detect biomacromolecules under physiological conditions can provide valuable information about structure-function relationship of GPCRs. This is of great significance for the in-depth study of GPCRs and the development of related drugs.

Analysis of β1- (A–E) and β2- (F–J) AR oligomerization and lateral mobility by single-molecule TIRF-M. Figure 1. Analysis of β1- (A–E) and β2- (F–J) AR oligomerization and lateral mobility by single-molecule TIRF-M. (Calebiro D, et al., 2013)

Our Services

We offer our customers with a comprehensive selection of single molecule analysis based on a wide variety of techniques to meet their different research needs.

Techniques Applications
Single molecule localization microscopy (SMLM)
  • Photoactivation localization microscopy (PALM)
  • Nanometer-scale resolution tools to visualize the spatial organization of GPCR complexes.
  • Quantitative analysis of GPCR monomers, dimers and oligomers.
  • Dissection of protein-protein interactions.
  • Fluorescence photoactivation localization microscopy (FPALM)
  • Stochastic optical reconstruction microscopy (STORM)
  • Dual-color photoactivation localization microscopy with photoactivatable dyes (PDPALM)
Single-molecule fluorescence microscopy (SMFM)
  • Single-molecule fluorescence resonance energy transfer (smFRET)
  • Conformational changes of GPCRs on time scales can be measured.
  • Real time monitoring of biomolecular structure dynamics.
  • Analysis of individual tracks of single molecules in parallel with high spatial precision.
  • Total internal reflection fluorescence microscopy (TIRFM)
Single-molecule force spectroscopy (SMFS)
  • Understand the relationship between molecular interactions and the structural stability of GPCRs.
  • Understand the effect of intra- and intermolecular interactions on the functional state of GPCRs.
Stimulated emission depletion (STED) microscopy
  • A super resolution microscopy technique for visualization of GPCR localization.
  • Live-imaging of GPCR trafficking in both cultured cells and tissues.
Structured illumination microscopy (SIM)
  • Super-resolution imaging of functional GPCRs.
  • Imaging the dynamics of live cells.

CD BioSciences provides our customers with customized single molecule analysis of GPCRs in a time- and cost-effective manner. Our experts will work closely with you to fully meet your requirements.

If you are interested in our services or have any specific needs, please feel free to contact us for more details.

References

  1. Calebiro D, et al. Single-molecule analysis of fluorescently labeled G-protein–coupled receptors reveals complexes with distinct dynamics and organization. Proceedings of the National Academy of Sciences, 2013, 110(2): 743-748.
  2. Jonas K C, et al. Single molecule analysis of functionally asymmetric G protein-coupled receptor (GPCR) oligomers reveals diverse spatial and structural assemblies. Journal of Biological Chemistry, 2015, 290(7): 3875-3892.