G Protein Dependent Functional Assays
Creative Biolabs provides G protein dependent functional assay services based on multiple assay formats to accelerate GPCR research and drug development. We can further help speed up your drug discovery by combining high-throughput technology.
Importance of G Protein Dependent Functional Assays
G proteins, also known as guanine nucleotide-binding proteins, typically exist in either an active GTP-bound state or an inactive GDP-bound state. G protein-coupled receptors (GPCRs), one of the most successful therapeutic targets, can activate heterotrimeric G proteins to elicit cellular responses. GPCR-G protein interactions are involved in signal transduction cascades and the modulation of downstream effector events.
Binding assays are helpful to identify new compounds targeting GPCRs. G protein dependent functional assays are essential for further analysis of G protein activation and G protein-mediated biological events, which can help to fully describe the overall characteristics of these compounds and provide useful information on the selection of candidate compounds.
Figure 1. The guanine nucleotide cycle of heterotrimeric G-proteins. (Johnston C A & Siderovski D P, 2007)
G Protein Dependent Functional Assay Services
We offer our customers with a comprehensive selection of G protein dependent functional assays to meet their different research needs.
|GTPγS binding assay||Gαi/o||
|cAMP assay||Gαs, Gαi/o||
|Reporter assay||Any GPCRs||
- Customized services based on multiple assays formats.
- One-stop solutions for all your problems.
- High-quality and reliable results under strict quality control conditions.
- Fast turnaround time and competitive price.
Creative Biolabs provides our customers with customized G protein dependent functional assay services in a time- and cost-effective manner. Our experts will work closely with you to fully meet your requirements.
If you are interested in our services or have any specific needs, please feel free to contact us for more details.
- ohnston C A & Siderovski D P. Receptor-mediated activation of heterotrimeric G-proteins: current structural insights. Molecular pharmacology, 2007, 72(2): 219-230.
- Mahoney J P & Sunahara R K. Mechanistic insights into GPCR–G protein interactions. Current opinion in structural biology, 2016, 41: 247-254.