GPCR Peptide Library
Creative Biolabs provides professional GPCR peptide library services for both GPCR research and drug development. We have developed a powerful peptide library construction platform to help our customers accelerate the understanding of GPCRs and promote the development of new drugs.
Brief Introduction
G protein-coupled receptor (GPCR), the largest family of membrane proteins in the human genome, is a large and versatile group of protein sensors which can respond to a diverse range of extracellular stimuli and assist in various different functions. Classical GPCR signaling is initiated by a ligand, such as endogenous peptides. It is of great significance to understand the receptor-ligand interactions and identify ligands at orphan GPCRs for the development of novel GPCR-targeted drugs.
Peptide library refers to a large collection of different peptides with a systematic combination of amino acids. The establishment of automated solid phase synthesis technologies and the advent of combinatorial chemistry make it possible to synthesize large numbers of peptides in large quantities. Peptide library is useful for drug discovery, screening and design.
Applications of Peptide Library
As a powerful tool for protein-related study, peptide library can be widely used in various popular research fields. We can provide customized peptide libraries for different downstream applications.
| Applications | |
|---|---|
| Antibody development and profiling | Antibody epitope mapping |
| Affinity optimization | Biological assay development |
| Biomarker identification | Enzyme motif discovery and profiling |
| Drug discovery and screening | Drug/proDrug design |
| Protein structure activity studies | Peptide mimetics |
| Protein conformation studies | Peptide drug carriers |
| Protein kinase studies | Protease studies |
| Receptor-ligand studies | Protein-protein interactions |
| Vaccine development | Identification of bioactive peptides |
| Screening for ligand-binding activities | More possibilities |
Types of Peptide Library
We provide multiple types of peptide libraries to meet different requirements of GPCR research and drug development. We have developed a variety of technical platforms, including but not limited to phage display, mRNA display, ribosome display, bacteria display, and yeast display.
| Library Type | Description |
|---|---|
| Alanine Peptide Scanning Library | Alanine is systematically substituted into each of the amino acid residues. Alanine peptide scanning library can be used to identify epitope activity and rapidly determine the contribution of each amino acid to the peptide function. |
| Overlapping Peptide Library | Overlapping peptide library can be used for continuous and linear epitope mapping, especially for the identification of T-cell and B-cell epitopes. |
| Positional Peptide Library | Selected positions in a peptide sequence are systematically replaced with all other natural amino acids. Positional peptide library is important for peptide sequence optimization. |
| Truncation Peptide Library | Truncation peptide library can be used to predict the shortest amino acid sequence for achieving optimal epitope activity. |
| T-cell Truncated Peptide Library | T-cell truncated peptide library can be used to test all possible T-cell epitopes across a protein of interest. |
| Random Peptide Library | Random peptide library is constructed by substituting selected positions with all 20 natural amino acids simultaneously. It can be used to elucidate potential substitutes for enhancing peptide activity. |
| Scrambled Peptide Library | Scrambled peptide library is constructed by carrying out permutation of the original peptide sequence. It can be used as negative control or random screening tool. |
Service Specifications
| Turnaround Time | Deliverable Format | Quality Documents | Price |
|---|---|---|---|
|
Lyophilized peptides in 96-well plate or individual vials |
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Online Inquiry |
Our Advantages
- Rigorous quality control and no cross-contamination.
- Comprehensive QC reports to ensure the highest quality and stability.
- Rapid high-throughput parallel platform.
- Various types of peptide libraries and customizable purity level.
- High throughput, fast turnaround and low cost.
- Strict confidentiality for the peptide sequences provided by our customers.
Creative Biolabs is always ready to provide the best services to each of our customers. Our experienced scientific team can provide customized services and one-stop solutions for your GPCR research and drug discovery.
If you are interested in our services or have any specific requirements, please feel free to contact us for more details.
References
- W Gruber C, et al. Ligand-based peptide design and combinatorial peptide libraries to target G protein-coupled receptors. Current pharmaceutical design, 2010, 16(28): 3071-3088.
- Foster S R, et al. Discovery of human signaling systems: Pairing peptides to G protein-coupled receptors. Cell, 2019, 179(4): 895-908. e21.