GPCR Identification
Creative Biolabs provides comprehensive and professional services for GPCR Identification to facilitate the cutting-edge programs of GPCR research and drug development.
Introduction of GPCR Identification
G protein-coupled receptors (GPCRs), the largest class of membrane proteins in human genome, are responsible for the intracellular communication and the sensory input by stimuli. Approximately one-third of approved therapeutics act by modulating GPCR function. However, the low abundance and intrinsic nature of GPCRs makes them difficult to analyze.
Protein, a complex macromolecule consisting of amino acids, is the essential part of organisms. Protein identification is an important work in biological research, disease diagnosis, and biopharmaceutics. The basic properties of proteins, such as molecular weight, isoelectric point, structure, biological function and immunological characteristics, can be identified and analyzed. The identification of GPCRs can provide valuable insights for further understanding of their basic properties and the regulation of their biological processes, thus greatly promoting drug discovery.
Figure 1. Principle of protein identification using MS/MS data. (Zhang W & Zhao X, 2013)
Our Services
With advanced techniques and experiences scientists, we can provide a series of services to enable GPCR identification in gel band, gel spot, solution or protein mixture. We guarantee to provide accurate and reliable results with fast turnaround time.
GPCR Identification in Gel Samples
1D gel and 2D gel samples with various types of staining, such as Colloidal Coomassie Blue, SYPRO Ruby, silver, and fluorescent dye, are accepted to identify GPCRs in gel bands or gel spots.
GPCR Identification in Solutions
GPCR ID in solutions can be used for applications such as affinity purification systems and monitoring protein expression. SDS-gel images should be submitted together with the samples to facilitate assessment of the total amount of proteins and dynamic range.
GPCR Identification in Mixture Samples
This service package is for GPCR ID from both simple and complex protein mixture samples. Sample types include cell lysate, tissue lysates, protein mixture, immunoprecipitation sample and pull-down sample.
Overview of Our Technology Platforms
| Technology Platforms | Description |
|---|---|
| Molecular mass determination | Molecular mass is the primary parameter in protein identification. Commonly used techniques include SDS-PAGE, exclusion chromatography, mass spectrometry, and NMR. |
| Isoelectric point determination | Protein isoelectric point is an important parameter for many analytical biochemistry and proteomics techniques. Isoelectrofocusing, chromatofocusing and ion exchange chromatography are commonly used methods. |
| Peptide mass fingerprinting | A frequently-used analytical technique for protein identification. Unknown proteins of interest are cleaved into smaller peptides to measure the absolute masses, which is then compared to the theoretical peptide masses of each protein encoded in the genome. |
| De novo peptide sequencing | Without prior knowledge of the amino acid sequence, the peptide sequences can be deduced using computational approaches. |
| Mass spectrometry | Mass spectrometry (MS) is a powerful analytical instrument and has become the primary method for protein identification from complex mixtures. |
| LC-MS and LC-MS/MS | LC-MS combines the physical separation capabilities of liquid chromatography or HPLC with the mass analysis capabilities of MS. 2D-LC-MS/MS and nano LC-MS/MS are more powerful analytical techniques to provide robust methods for proteomic analysis and pharmaceutical bioanalysis. |
| GPCR structural biology | Protein crystallization, structure determination by X-ray crystallography, and nuclear magnetic resonance (NMR) spectroscopy are the main methods to determine high resolution protein structure. |
| GPCR proteomics | Proteomics, the large-scale study of proteomes, has been able to identify the ever-increasing numbers of proteins. The comprehensive proteomic characterization of GPCRs is important for future drug discovery. |
Our Advantages
- Ultra-high sensitivity and reliability
- Ability to Identify GPCR in multiple sample types
- Guaranteed success
- A strong track record
- Fast turnaround time and competitive price
Creative Biolabs is always ready to provide the best services to each of our customers. We provide a complete protein profiling service to help our customers get a complete analysis of their protein samples. If you are interested in our services or have any specific requirements, please feel free to contact us for more details.
References
- Zhang W & Zhao X. Method for rapid protein identification in a large database. BioMed research international, 2013, 2013.
- Gevaert K & Vandekerckhove J. Protein identification methods in proteomics. ELECTROPHORESIS: An International Journal, 2000, 21(6): 1145-1154.
- Lin D, et al. Large-scale protein identification using mass spectrometry. Biochimica et Biophysica Acta (BBA)-Proteins and Proteomics, 2003, 1646(1-2): 1-10.