CD BioSciences has the expertise in providing a variety of mutagenesis services for GPCR research and drug development.
Introduction of GPCR Mutagenesis
Mutagenesis refers to the process of spontaneous or induced genetic mutation. Spontaneous mutation may lead to irreversible effects, such as cancer and various heritable diseases, but it is also an essential step of natural selection. With the development of science, mutagenesis, a group of techniques that can be used to change DNA sequence at the molecular level, has become an important tool for biological research and drug development.
G protein-coupled receptors (GPCRs) are a large superfamily of high flexible and dynamic membrane proteins. They are involved in the transmission of extracellular signals and the regulation of intracellular signaling pathways, and induce a range of cellular and physiological responses. Mutations of GPCRs are associated with the pathogenesis of a wide range of diseases. Therefore, mutagenesis can be used as a powerful tool to analyze the structure-function relationship of disease-causing mutations in detail.
Figure 1. Mutations alter the ability of the receptor to transduce the signal. (Stoy H & Gurevich V V., 2015)
GPCR Mutagenesis Services
We provide multiple GPCR mutagenesis services for different research purposes. Our experts are dedicated to helping you find answers for your research and accelerate your project lifecycle.
Site-directed mutagenesis (SDM), or oligonucleotide-directed mutagenesis, is an important technique to alter a gene or vector sequence at a selected location. SDM has been widely used in GPCR research to understand many of their functions.
Our SDM services include point mutations, insertions and deletions. We guarantee 100% accuracy of obtained mutant DNA clones. Our high-quality services are applicable for any downstream application.
Random mutagenesis refers to the introduction of point mutations at random positions through the use of chemical mutagens, error-prone PCR, saturation mutagenesis or mutator strains.
Our random mutagenesis services can create combinatorial libraries and generate genetic diversity. We can help our customers create a preliminary map of GPCR functional domains.
Mutant Libraries, which represent protein variants, have been widely used in the identification of structurally and functionally critical residues and the optimization of protein function.
We have strong expertise and state-of-the-art technology in mutant library construction. We have the ability to construct multiple types of mutant libraries, such as site-directed mutagenesis libraries, site-directed saturation mutagenesis libraries, sequential permutation scanning libraries, randomized and degenerated libraries, and precision mutant libraries.
CD BioSciences provides customized GPCR mutagenesis services in a fast and cost-saving way. If you are interested in our services or have any specific needs, please feel free to contact us for more details.
- Stoy H & Gurevich V V. How genetic errors in GPCRs affect their function: Possible therapeutic strategies. Genes & diseases, 2015, 2(2): 108-132.
- Conner A C, et al. The use of site-directed mutagenesis to study GPCRs. Receptor Signal Transduction Protocols. Humana Press, Totowa, NJ, 2011: 85-98.