GPCR Expression
Creative Biolabs provides our customers with comprehensive solutions for GPCR expression to facilitate their cutting-edge programs.
Importance of GPCR Expression
G protein-coupled receptors (GPCRs), the largest family of cell surface receptors, play a pivotal role in signal transduction and are involved in numerous physiological regulatory mechanisms in human. As one of the largest classes of pharmacological therapeutic targets, the detailed structural and functional knowledge of GPCRs is of great importance for understanding drug-target interaction.
Most GPCRs are naturally found only in very little amounts. The inherent structural flexibility and dynamic characteristics of GPCRs are also the main obstacles. Thus, it is indispensable to establish a robust, efficient and reliable expression system to obtain stable GPCRs in sufficient quantities for various downstream applications, such as basic functional research, structural studies, antibody development, and drug discovery. Highly expressed GPCRs also have great potential in developing novel therapies for a wide range of diseases.
Figure 1. Simplified flow-chart of the production strategy of GPCRs. (Maeda S & Schertler G F X, 2013)
Comprehensive Solutions for GPCR Expression
We provide comprehensive solutions for GPCR expression based on various strategies and technique platforms. Our experienced scientific team can select the most suitable expression system and provide customized services tailored to specific requirements of our customers.
Expression System | Advantages |
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Cell-based expression system |
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Cell-free expression system |
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Transient expression system |
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Stable expression system |
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Bacterial expression system - E. coli - Photosynthetic bacteria - Lactococcus lactis |
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Yeast expression system - Saccharomyces cerevisiae - Pichia pastoris |
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Insect cell-based expression system - Sf9 cells - Sf21 cells |
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Mammalian cell expression system - CHO cells - HEK cells - Rabbit Reticulocyte Lysate |
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Wheat germ expression system |
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Plant expression system |
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Large-scale expression system - cGMP - GLP |
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If you are interested in our GPCR expression services or have any specific needs, please feel free to contact us for more details.
References
- Maeda S & Schertler G F X. Production of GPCR and GPCR complexes for structure determination. Current opinion in structural biology, 2013, 23(3): 381-392.
- Insel P A, et al. GPCR expression in tissues and cells: are the optimal receptors being used as drug targets?. British journal of pharmacology, 2012, 165(6): 1613-1616.
- Milić D & Veprintsev D B. Large-scale production and protein engineering of G protein-coupled receptors for structural studies. Frontiers in pharmacology, 2015, 6: 66.