Specification

• Detection Method:
• Fluorescent

• Component:
• • FRET B/G substrate,
  • DMSO (for Solution A)
  • Lysis Buffer
  Each 15 ml kit is sufficient for 3,750 × 40 µl standard protocol well assays.

• Storage:
• • FRET B/G Substrate should be stored at -20°C. Desiccated and protected from light.
  • DMSO (for Solution A) and Lysis Buffer should be stored at 22-25°C, protected from direct light.

• Applicable Equipment:
• Fluorometer, Microplate Reader

• Substrate:
• FRET B/G

• Substrate Properties:
• Chemical Substrate

• Substrate Type:
• Beta-Lactamase Substrate

Description

• The FRET B/G Kit (10×) provides a ratiometric, end-point method of analyzing beta-lactamase activity in cell lysates. It is a very simple assay with minimized experimental noise by combining the components for lysis and detection in a single reagent addition. This kit can be used with both top-read or bottom read fluorescence instrumentation.