CHO-K1/Gα15-NFAT-bla/C5aR1 Division Arrested Assay Kit

CHO-K1/Gα15-NFAT-bla/C5aR1 Division Arrested Assay Kit

For Research Use Only. Not For Clinical Use.

Catalog Number: DAC0005

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  • Cell Line:
  • CHO-K1
  • GPCR Family:
  • Complement Peptide (Anaphylotoxin) Receptors
  • Gene Name:
  • C5AR1
  • GenBank Accession Number:
  • NM_001736.2
  • Cell State:
  • Division-Arrested Cells
  • Detection Method:
  • Fluorescent
  • Component:
  • This assay kit includes sufficient cells and substrate to analyze 1 × 384-well plate
  • Shipping Condition:
  • Dry Ice
  • Storage:
  • Should be stored in liquid nitrogen immediately upon receipt.
  • Population Status:
  • Single Cell Clone Origin
  • Receptor Type:
  • BLA (Beta-Lactamase)


  • CHO-K1/Gα15-NFAT-bla/C5aR1 is a stable recombinant human cell line expressing the human Complement Component 5a Receptor 1 (C5aR1). Under the control of the Nuclear Factor of Activated T-cells (NFAT) response element and a promiscuous G Protein, Gα15, this cell line can stably express the beta-lactamase (bla) reporter gene. Using a low-dose treatment of Mitomycin-C, these cells are irreversibly division arrested, but without apparent toxicity or change in cellular signal transduction. These cells have been functionally validated and tested under variable conditions.