Specification

• Cell Line:
• CHO-K1

• GPCR Family:
• Complement Peptide (Anaphylotoxin) Receptors

• Gene Name:
• C5AR1

• GenBank Accession Number:
• NM_001736.2

• Cell State:
• Division-Arrested Cells

• Detection Method:
• Fluorescent

• Component:
• This assay kit includes sufficient cells and substrate to analyze 1 × 384-well plate

• Shipping Condition:
• Dry Ice

• Storage:
• Should be stored in liquid nitrogen immediately upon receipt.

• Population Status:
• Single Cell Clone Origin

• Receptor Type:
• BLA (Beta-Lactamase)

Description

• CHO-K1/Gα15-NFAT-bla/C5aR1 is a stable recombinant human cell line expressing the human Complement Component 5a Receptor 1 (C5aR1). Under the control of the Nuclear Factor of Activated T-cells (NFAT) response element and a promiscuous G Protein, Gα15, this cell line can stably express the beta-lactamase (bla) reporter gene. Using a low-dose treatment of Mitomycin-C, these cells are irreversibly division arrested, but without apparent toxicity or change in cellular signal transduction. These cells have been functionally validated and tested under variable conditions.