U2OS/ADORA1-bla Division Arrested Assay Kit

U2OS/ADORA1-bla Division Arrested Assay Kit

For Research Use Only. Not For Clinical Use.

Catalog Number: DAC0027

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1 kit Online Inquiry


  • Cell Line:
  • U2OS
  • GPCR Family:
  • Adenosine Receptors
  • Gene Name:
  • ADORA1
  • GenBank Accession Number:
  • NM_000674.1
  • Cell State:
  • Division-Arrested Cells
  • Detection Method:
  • Fluorescent
  • Component:
  • This assay kit includes sufficient cells and substrate to analyze 1 × 384-well plate
  • Shipping Condition:
  • Dry Ice
  • Storage:
  • Should be stored in liquid nitrogen immediately upon receipt.
  • Population Status:
  • Pool of Stable Cells
  • Receptor Type:
  • BLA (Beta-Lactamase)
  • Cytoplasmic Tail:
  • Tail from AVPR2 GPCR


  • U2OS/ADORA1-bla is a stable recombinant human cell line expressing the human Adenosine A1 receptor (ADORA1) linked to a TEV protease site and a Gal4-VP16 transcription factor. Under the control of a UAS response element, this cell line can stably express a beta-arrestin/TEV protease fusion protein and the beta-lactamase (bla) reporter gene. Using a low-dose treatment of Mitomycin-C, these cells are irreversibly division arrested, but without apparent toxicity or change in cellular signal transduction. These cells have been functionally validated and tested under variable conditions.