Specification

• Cell Line:
• U2OS

• GPCR Family:
• Adenosine Receptors

• Gene Name:
• ADRA2A

• GenBank Accession Number:
• NM_000681.2

• Cell State:
• Division-Arrested Cells

• Detection Method:
• Fluorescent

• Component:
• This assay kit includes sufficient cells and substrate to analyze 1 × 384-well plate

• Shipping Condition:
• Dry Ice

• Storage:
• Should be stored in liquid nitrogen immediately upon receipt.

• Population Status:
• Single Cell Clone Origin

• Receptor Type:
• BLA (Beta-Lactamase)

• Cytoplasmic Tail:
• Tail from AVPR2 GPCR

Description

• U2OS/ADRA2A-bla is a stable recombinant human cell line expressing the human Adrenergic, Alpha-2A-, Receptor (ADRA2A) linked to a TEV protease site and a Gal4-VP16 transcription factor. Under the control of a UAS response element, this cell line can stably express a beta-arrestin/TEV protease fusion protein and the beta-lactamase (bla) reporter gene. Using a low-dose treatment of Mitomycin-C, these cells are irreversibly division arrested, but without apparent toxicity or change in cellular signal transduction. These cells have been functionally validated and tested under variable conditions.