Specification

• Cell Line:
• HEK293T

• GPCR Family:
• Lysophosphatidic Acid (LPA) Receptors

• Gene Name:
• EDG3

• Assay Target Class:
• GPCR

• GenBank Accession Number:
• NM_005226.2

• Detection Method:
• Fluorescent

• Population Status:
• Single Cell Clone Origin

• Shipping Condition:
• Dry Ice

• Storage:
• Should be stored in liquid nitrogen immediately upon receipt.

• Technique:
• FRET

Description

• We have different stable recombinant cell lines that express a variety of GPCRs for binding and functional assays.

Detailed Information

• HEK293/Gα15-NFAT-bla/EDG3 is a stable recombinant human cell line expressing the human Endothelial Differentiation Receptor 3 (EDG3). Under the control of the Nuclear Factor of Activated T-cells (NFAT) response element and the Promiscuous G protein, Gα15, this cell line can stably express the beta-lactamase (bla) reporter gene. This cell line has been functionally validated and tested under variable conditions. EDG3 or Sphingosine 1-phosphate receptor 3 (S1P3), which is a Gq/Gi/Go/Gα13/Gα12 coupled GPCR, has been shown identified as a functional receptor for sphingosine 1-phosphate and likely contributes to the regulation of angiogenesis and vascular endothelial cell function.