GPCR Stable Cell Lines
Stable cell lines are widely used in many important applications, including the study on gene function, the identification of biological mechanisms, and the biological product manufacturing (such as monoclonal antibodies and recombinant proteins). In pharmaceutical industry, stable cells lines can be used from target discovery to compound screening. G protein-coupled receptors (GPCRs), also known as seven-(pass)-transmembrane domain receptors or 7TM receptors, are a large class of important therapeutic targets. Therefore, GPCR stable cell lines are quite useful tools for GPCR research and drug discovery.
The process of developing stable cell lines usually begins with the transfer of the required plasmids to the selected host cells. The generation of stable cell lines is a time-consuming process. Taking fully advantage of our time-tested expertise in stable cell line development, GPCR biology and drug discovery, Creative Biolabs can develop various GPCR stable cell lines expressing various members of GPCR family to help our customers save time and cost.
Parental Cell List
We have successfully developed GPCR stable cell lines in many types of parental cells, including but not limited to the following list.
| 1321N1 | 2F-2B | 4T1 | 786O |
| A549 | CHO | H1299 | HEK293 |
| HEK293F | HEK293T | HepG2 | Jurkat |
| MCF7 | THP-1 | U2OS | V79 |
GPCR List
We have successfully developed stable cell lines expressing various members of GPCR family, including but not limited to the following list.
| Receptor Family | Receptor | Receptor Family | Receptor |
|---|---|---|---|
| 5-HT | 5-HT1A | Melanin-Concentrating Hormone | MCH1 |
| 5-HT1B | MCH2 | ||
| 5-HT2A | Melanocortin | MC1 | |
| 5-HT2B | MC2 | ||
| 5-HT3C | MC3 | ||
| 5-HT4A | MC4 | ||
| 5-HT4B | Melatonin | MT1 | |
| 5-HT4D | MT2 | ||
| 5-HT6 | Metabotropic Glutamate | mGlu1 | |
| 5-HT7 | mGlu2 | ||
| Adenosine | A1 | mGlu3 | |
| A2A | mGlu4 | ||
| A2B | mGlu5 | ||
| A3 | mGlu6 | ||
| Adrenergic | α1A | mGlu7 | |
| α1B | mGlu8 | ||
| α1D | Motilin | Motilin | |
| α2A | Muscarinic | M1 | |
| α2B | M2 | ||
| α2C | M3 | ||
| β1 | M4 | ||
| β2 | M5 | ||
| β3 | Neuromedin U | NMU1 | |
| Angiotensin | AT1 | NMU2 | |
| AT2 | Neuropeptide | NPBW1 | |
| Apelin | APJ | NPBW2 | |
| Bombesin | BB1 | NPFF1 | |
| BB2 | NPFF2 | ||
| BB3 | NPS | ||
| Bradykinin | B1 | NPS(2) | |
| B2 | NPSIle107 | ||
| Calcitonin | AM1 | Y1 | |
| AM2 | Y2 | ||
| AMY1 | Y4 | ||
| AMY2 | Y5 | ||
| AMY3 | Neurotensin | NTS1 | |
| CGRP | NTS2 | ||
| Calcium-Sensing | CaSR | Opioid | δ Opioid |
| Cannabinoid | CB1 | κ Opioid | |
| CB1a | μ Opioid | ||
| CB1b | NOP | ||
| CB2 | Orexin | OX1 | |
| Chemokine | CCR1 | OX2 | |
| CCR2 | Orphan | GPR3 | |
| CCR2b | GPR119 | ||
| CCR3 | GPR139 | ||
| CCR4 | GPR142 | ||
| CCR5 | GPR151 | ||
| CCR6 | GPR17 | ||
| CCR7 | GPR18 | ||
| CCR8 | GPR183 | ||
| CCR9 | GPR27 | ||
| CCR10 | GPR34 | ||
| CXCR1 | GPR35 | ||
| CXCR2 | GPR37 | ||
| CXCR3 | GPR37L1 | ||
| CXCR4 | GPR39 | ||
| CXCR5 | GPR50 | ||
| CXCR6 | GPR55 | ||
| CXCR7 | GPR84 | ||
| Cholecystokinin | CCK1 | GPR85 | |
| CCK2 | MAS1 | ||
| Corticotrophin-Releasing Factor | CRF1 | MRGX1 | |
| CRF2 | MRGX2 | ||
| Dopamine | D1 | Parathyroid Hormone | PTH1 |
| D2 | PTH2 | ||
| D3 | Platelet-Activating Factor | PAF | |
| D4 | Prokineticin | PK1 | |
| D5 | PK2 | ||
| Endothelin | ETA | Prolactin-Releasing Peptide | PrRP |
| ETB | Prostanoid | DP1 | |
| Formyl | FPR1 | DP2 | |
| FPR2 | FP | ||
| Free Fatty Acid | GPR40 | IP1 | |
| GPR41 | Prostaglandin E1 | ||
| GPR43 | Prostaglandin E2 | ||
| GPR84 | Prostaglandin E3 | ||
| GPR119 | Prostaglandin E4 | ||
| GPR120 | TP | ||
| GPR120L | Protease-Activated | PAR1 | |
| Galanin | GAL1 | PAR2 | |
| GAL2 | PAR4 | ||
| Ghrelin | GHS-R1a | Purinergic | P2Y1 |
| Glucagon | GHRH | P2Y2 | |
| GIP | P2Y4 | ||
| GLP-1 | P2Y6 | ||
| GLP-2 | P2Y11 | ||
| Gonadotrophin-Releasing Hormone | GnRH | P2Y12 | |
| Histamine | Histamine1 | P2Y13 | |
| Histamine2 | P2Y14 | ||
| Histamine3 | Relaxin | RXFP1 | |
| Histamine4 | RXFP2 | ||
| Hydroxycarboxylic Acid | HCA1 | RXFP3 | |
| HCA2 | Somatostatin | SST1 | |
| HCA3 | SST2 | ||
| Kisspeptin | GPR54 | SST3 | |
| Leukotriene | BLT1 | SST4 | |
| BLT2 | SST5 | ||
| CysLT1 | Tachykinin | NK1 | |
| CysLT2 | NK2 | ||
| Lysophospholipid | LPA1 | NK3 | |
| LPA2 | Thyrotropin-Releasing Hormone | TRH | |
| LPA3 | Trace Amine | TA1 | |
| LPA4 | Urotensin | Urotensin-Ⅱ | |
| LPA5 | Vasoactive Intestinal Peptide | PAC1 | |
| S1P1 | VPAC1 | ||
| S1P2 | VPAC2 | ||
| S1P3 | Vasopressin | V1A | |
| S1P4 | V1B | ||
| S1P5 | V2 |
Advantages
- Improved cell surface expression.
- Comprehensive coverage of GPCR targets.
- Flexible cell types and species.
- Applicable to high-throughput screening and profiling, and better signal-to-noise ratio.
- Customized services and ready-to-use cell lines.
- Available backup cell lines.
- Competitive price and fast turnaround time.
Creative Biolabs provides highly validated GPCR stable cell lines to help our customers make GPCR research easy and standard. Our experienced scientists can customize GPCR stable cell lines according to your specific needs to enable your discovery programs.
If you need more detailed information, please don't hesitate to contact us.